pIRESneo2 mycBioID-Tollip
(Plasmid #178054)

• Purpose: myc-BioID fused to human Tollip cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.
• Depositing Lab: David Tumbarello
• Publication: Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi: 10.15252/embj.2019102539. Epub 2020 Apr 20.

Backbone

• Vector backbone: pIRESneo2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5300
• Total vector size (bp): 7140
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Toll-Interacting Protein
• Alt name: IL-1RAcPIP
• Species: H. sapiens (human)
• Insert Size (bp): 1840
• GenBank ID: NM_019009.4
• Entrez Gene: TOLLIP (a.k.a. IL-1RAcPIP)
• Promoter: CMV
• Tag / Fusion Protein:
  • mycBioID (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV FWD
• 3′ sequencing primer: IRES REV

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry