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PB CAG-L-bpA-2xSV40pA-L-tdGFP-Pten_shRNAmir
(Plasmid #178044)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178044 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Custom
  • Backbone manufacturer
    Hyung-song Nam, Capecchi laboratory
  • Vector type
    Mammalian Expression, RNAi, Cre/Lox ; piggyBac

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pten_1523 shRNAmir
  • Species
    Synthetic

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    piggyBac ITR's were synthesized from sequences in the NCBI database. CAG promoter is from pCAGGS obtained from BCCM/LMBP. bGH pA is from Neal Copeland. SV40 pA is from Clontech. sfGFP is from Sandia Biotech. Backbone is from Stratagene.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

mir-30a "miR-E" shRNAmir expression cassette sequence was obtained from Fellmann et al., 2013 (https://doi.org/10.1016/j.celrep.2013.11.020) and synthesized.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB CAG-L-bpA-2xSV40pA-L-tdGFP-Pten_shRNAmir was a gift from Mario Capecchi (Addgene plasmid # 178044 ; http://n2t.net/addgene:178044 ; RRID:Addgene_178044)
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