pET22b-T5-sfGFP*-XcP4H-MH4R
(Plasmid #178043)

• Purpose: Express green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTP
• Depositing Lab: Han Xiao
• Publication: Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 10.1016/j.chempr.2020.07.013. Epub 2020 Aug 12.

Backbone

• Vector backbone: pET
• Backbone size w/o insert (bp): 5130
• Total vector size (bp): 8509
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: superfolder green fluorescent protein with TAG at 151 position
• Insert Size (bp): 741
• Tag / Fusion Protein:
  • His tag (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: XcP4H with W179F mutation
• Alt name: phenylalanine 4-hydroxylases with from W179F mutation
• Species: Xanthomonas campestris
• Insert Size (bp): 891

Gene/Insert 3

• Gene/Insert name: dihydropterine reductase from human
• Insert Size (bp): 735

Gene/Insert 4

• Gene/Insert name: pterin-4a-carbinolamine dehydratase from human
• Insert Size (bp): 316

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry