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Addgene

pUltra-5HTP
(Plasmid #178042)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178042 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUltra
  • Backbone size w/o insert (bp) 4067
  • Total vector size (bp) 5362
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Modified ScTrpRS for 5HTP incoporation
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1299
  • Mutation
    T107C, P254T, C255A

Gene/Insert 2

  • Gene/Insert name
    suppressor tRNA for 5HTP incorporation
  • Species
    S. cerevisiae (budding yeast)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUltra-5HTP was a gift from Han Xiao (Addgene plasmid # 178042 ; http://n2t.net/addgene:178042 ; RRID:Addgene_178042)

  • For your References section:

    Creation of Bacterial cells with 5-Hydroxytryptophan as a 21(st) Amino Acid Building Block. Chen Y, Tang J, Wang L, Tian Z, Cardenas A, Fang X, Chatterjee A, Xiao H. Chem. 2020 Oct 8;6(10):2717-2727. doi: 10.1016/j.chempr.2020.07.013. Epub 2020 Aug 12. 10.1016/j.chempr.2020.07.013 PubMed 33102928
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