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Psicheck Cdk2ap1Exon1
(Plasmid #178028)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178028 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psiCHECK-2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 6273
  • Total vector size (bp) 6251
  • Modifications to backbone
    None
  • Vector type
    Mammalian Expression, Luciferase ; Nonviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cdk2ap1
  • Alt name
    Cdk2ap1 Canonical
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    257
  • GenBank ID
    13445 13445
  • Entrez Gene
    Cdk2ap1 (a.k.a. Apc10, Cdkap1, DORC1, Doc1, ST19, doc-1, p12)
  • Promoter Cloned in
  • Tag / Fusion Protein
    • Luciferase (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TTAATACGACTCACTATAGGgcggccgcccctgcgccgcg
  • 3′ sequencing primer TCGTACACCTTGGAAGCCATccccgcgggcggcgggcgcg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Psicheck Cdk2ap1Exon1 was a gift from Lin He (Addgene plasmid # 178028 ; http://n2t.net/addgene:178028 ; RRID:Addgene_178028)

  • For your References section:

    A mouse-specific retrotransposon drives a conserved Cdk2ap1 isoform essential for development. Modzelewski AJ, Shao W, Chen J, Lee A, Qi X, Noon M, Tjokro K, Sales G, Biton A, Anand A, Speed TP, Xuan Z, Wang T, Risso D, He L. Cell. 2021 Oct 7. pii: S0092-8674(21)01104-1. doi: 10.1016/j.cell.2021.09.021. 10.1016/j.cell.2021.09.021 PubMed 34644528
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