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pM-ErbB4-delta-kinase-PY1 mutant
(Plasmid #17801)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17801 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pM
  • Backbone manufacturer
    BD Biosciences
  • Backbone size w/o insert (bp) 3500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ErbB4
  • Alt name
    HER4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    920
  • Mutation
    Carboxy-terminal fragment, amino acids 988-1292, kinase domain deleted, Tyrosine 1035 changed to Alanine
  • GenBank ID
    NM_001042599
  • Entrez Gene
    ERBB4 (a.k.a. ALS19, HER4, p180erbB4)
  • Tag / Fusion Protein
    • GAL4-BD (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pM-ErbB4-delta-kinase-PY1 mutant was a gift from Marius Sudol (Addgene plasmid # 17801 ; http://n2t.net/addgene:17801 ; RRID:Addgene_17801)

  • For your References section:

    WW domain-containing protein YAP associates with ErbB-4 and acts as a co-transcriptional activator for the carboxyl-terminal fragment of ErbB-4 that translocates to the nucleus. Komuro A, Nagai M, Navin NE, Sudol M. J Biol Chem. 2003 Aug 29. 278(35):33334-41. 10.1074/jbc.M305597200 PubMed 12807903
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