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Addgene

sm-9xGCN4-Renilla-MS2
(Plasmid #178009)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178009 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSF4
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sm-9xGCN4
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGAGGTGGAGGTTCTGGAGGAGAAGAACTTTTGAGCAAGAATT
  • 3′ sequencing primer GCTGACCGGTGCGGCCGCTGGCCTGAGCCTGAGCCCTTTT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sm-9xGCN4-Renilla-MS2 was a gift from Jeffrey Chao (Addgene plasmid # 178009 ; http://n2t.net/addgene:178009 ; RRID:Addgene_178009)

  • For your References section:

    Live imaging of the co-translational recruitment of XBP1 mRNA to the ER and its processing by diffuse, non-polarized IRE1alpha. Gomez-Puerta S, Ferrero R, Hochstoeger T, Zubiri I, Chao J, Aragon T, Voigt F. Elife. 2022 Jun 22;11. pii: 75580. doi: 10.7554/eLife.75580. 10.7554/eLife.75580 PubMed 35730412
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