pcDNA5-FRT/TO-N-mEGFP-PARP6
(Plasmid
#178005)
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PurposeExpresses N-terminally mEGFP-tagged PARP6
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 178005 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA5-FRT/TO-N-mEGFP
- Total vector size (bp) 7815
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePARP6
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Alt namepoly(ADP-ribose) polymerase family member 6
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SpeciesH. sapiens (human)
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Insert Size (bp)1896
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Entrez GenePARP6 (a.k.a. ARTD17, PARP-6-B1, PARP-6-C, pART17)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EGFP C
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2022.02.22.481411 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5-FRT/TO-N-mEGFP-PARP6 was a gift from Karla Feijs (Addgene plasmid # 178005 ; http://n2t.net/addgene:178005 ; RRID:Addgene_178005) -
For your References section:
Protein and RNA ADP-ribosylation detection is influenced by sample preparation and reagents used. Weixler L, Ikenga NJ, Voorneveld J, Aydin G, Bolte TM, Momoh J, Butepage M, Golzmann A, Luscher B, Filippov DV, Zaja R, Feijs KL. Life Sci Alliance. 2022 Nov 11;6(1):e202201455. doi: 10.26508/lsa.202201455. 10.26508/lsa.202201455 PubMed 36368907