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pcDNA5-FRT/TO-N-mEGFP-PARP15
(Plasmid #178000)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178000 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO/N-mEGFP
  • Total vector size (bp) 7956
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PARP15
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2074
  • Entrez Gene
    PARP15 (a.k.a. ARTD7, BAL3, pART7)
  • Tag / Fusion Protein
    • mEGFP (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.02.22.481411 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-FRT/TO-N-mEGFP-PARP15 was a gift from Karla Feijs (Addgene plasmid # 178000 ; http://n2t.net/addgene:178000 ; RRID:Addgene_178000)

  • For your References section:

    Protein and RNA ADP-ribosylation detection is influenced by sample preparation and reagents used. Weixler L, Ikenga NJ, Voorneveld J, Aydin G, Bolte TM, Momoh J, Butepage M, Golzmann A, Luscher B, Filippov DV, Zaja R, Feijs KL. Life Sci Alliance. 2022 Nov 11;6(1):e202201455. doi: 10.26508/lsa.202201455. 10.26508/lsa.202201455 PubMed 36368907
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