pBABE-neo largeTcDNA
(Plasmid #1780)

• Purpose: Retroviral expression of Large T Antigen. Used to create immortalized cell lines.
• Depositing Lab: Bob Weinberg
• Publication: Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Backbone

• Vector backbone: pBABE-neo
• Backbone size w/o insert (bp): 5330
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: large T antigen
• Alt name: large T cDNA
• Alt name: pBNT
• Alt name: LTc
• Species: simian virus
• Insert Size (bp): 2200
• Entrez Gene: SV40gp6 (a.k.a. SV40gp6)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE-3

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 22 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

SspI site in Amp gene.