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pDEST17-DXO
(Plasmid #177993)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177993 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST17
  • Total vector size (bp) 5893
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DXO
  • Alt name
    Decapping and exoribonuclease protein
  • Alt name
    DOM3Z
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1195
  • Entrez Gene
    DXO (a.k.a. DOM3L, DOM3Z, NG6, RAI1)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.02.22.481411 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST17-DXO was a gift from Karla Feijs (Addgene plasmid # 177993 ; http://n2t.net/addgene:177993 ; RRID:Addgene_177993)

  • For your References section:

    Protein and RNA ADP-ribosylation detection is influenced by sample preparation and reagents used. Weixler L, Ikenga NJ, Voorneveld J, Aydin G, Bolte TM, Momoh J, Butepage M, Golzmann A, Luscher B, Filippov DV, Zaja R, Feijs KL. Life Sci Alliance. 2022 Nov 11;6(1):e202201455. doi: 10.26508/lsa.202201455. 10.26508/lsa.202201455 PubMed 36368907
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