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p2xFLAGhYAP2-WW1-mutant
(Plasmid #17795)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17795 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p2xFLAG-CMV2
  • Backbone size w/o insert (bp) 4662
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Yes-kinase associated protein
  • Alt name
    YAP2, YAP65 WW1-mutant
  • Alt name
    YAP2 isoform, WW1-mutant
  • Alt name
    YAP2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Mutation
    Tryptophan 199 to Alanine; Proline 202 to Alanine
  • GenBank ID
    NM_006106
  • Entrez Gene
    YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
  • Tag / Fusion Protein
    • 2xFlag (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

YAP was cloned in my lab

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2xFLAGhYAP2-WW1-mutant was a gift from Marius Sudol (Addgene plasmid # 17795 ; http://n2t.net/addgene:17795 ; RRID:Addgene_17795)

  • For your References section:

    WW domain-containing protein YAP associates with ErbB-4 and acts as a co-transcriptional activator for the carboxyl-terminal fragment of ErbB-4 that translocates to the nucleus. Komuro A, Nagai M, Navin NE, Sudol M. J Biol Chem. 2003 Aug 29. 278(35):33334-41. 10.1074/jbc.M305597200 PubMed 12807903
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