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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17793 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep2xFLAG-CMV2
- Backbone size w/o insert (bp) 4662
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameYes-kinase associated protein
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Alt nameYAP2, YAP65
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Alt nameYAP2 isoform, wild type
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SpeciesH. sapiens (human)
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Insert Size (bp)1600
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GenBank IDNM_006106
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Entrez GeneYAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
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Tag
/ Fusion Protein
- 2xFlag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
YAP was cloned in my lab
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p2xFLAGhYAP2 was a gift from Marius Sudol (Addgene plasmid # 17793 ; http://n2t.net/addgene:17793 ; RRID:Addgene_17793) -
For your References section:
WW domain-containing protein YAP associates with ErbB-4 and acts as a co-transcriptional activator for the carboxyl-terminal fragment of ErbB-4 that translocates to the nucleus. Komuro A, Nagai M, Navin NE, Sudol M. J Biol Chem. 2003 Aug 29. 278(35):33334-41. 10.1074/jbc.M305597200 PubMed 12807903