p2B-58
(Plasmid
#177928)
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PurposeExpresses yeGFP under control of doxycycline-responsive TET4 promoter (contains four rtTA binding sites)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 177928 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS426
- Backbone size w/o insert (bp) 5726
- Total vector size (bp) 7098
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEGFP
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Alt namepTET4_yeGFP
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SpeciesSynthetic
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Insert Size (bp)1378
- Promoter TET4 (contains four rtTA binding sites)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CGTAATACGACTCACTATAG
- 3′ sequencing primer CAATTAACCCTCACTAAAGG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byMads Kaern (University of Ottawa) kindly provided DNA sequences to design the inserts, as described in Roney et al. 2016 Sci Rep.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please refer to Roney et al. 2016 Sci Rep. "Improvement of the reverse tetracycline transactivator by single amino acid substitutions that reduce leaky target gene expression to undetectable levels" (doi: 10.1038/srep27697) for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p2B-58 was a gift from Vadim Gladyshev (Addgene plasmid # 177928 ; http://n2t.net/addgene:177928 ; RRID:Addgene_177928) -
For your References section:
Improved reverse tetracycline transactivator plasmids. Barre B, Gladyshev V. (unpublished) PubMed 27323850