pCEP4-puroeGFP
(Plasmid #177922)

• Purpose: Constitutively expresses eGFP with puromycin selection
• Depositing Lab: Wesley Sundquist
• Publication: Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(22)00240-8. doi: 10.1016/j.cub.2022.02.018.

Backbone

• Vector backbone: pCEP4
• Backbone size w/o insert (bp): 10188
• Total vector size (bp): 10586
• Modifications to backbone: Replaced hygromycin resistance with puromycin resistance
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: eGFP
• Species: Synthetic
• Insert Size (bp): 720
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV

Resource Information

• Supplemental Documents:
  • pCEP4-puroeGFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note Addgene's NGS result contains several ambiguous bases in the EBNA1 feature. NGS routinely has a difficult time sequencing through this element. Depositor confirms EBNA1 is functional.