p2xFLAGhYAP1-WW mutant
(Plasmid #17792)

• Depositing Lab: Marius Sudol
• Publication: Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-41.

Backbone

• Vector backbone: p2xFLAG-CMV2
• Backbone size w/o insert (bp): 4662
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Yes-kinase associated protein
• Alt name: YAP1, YAP65
• Alt name: YAP1 isoform, mutant
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• Mutation: Tryptophan 199 to Alanine; Proline 202 to Alanine
• GenBank ID: NM_006106
• Entrez Gene: YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
• Tag / Fusion Protein:
  • 2xFlag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: N/A

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

YAP was cloned in my lab