pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 4-2
(Plasmid #177919)

• Purpose: Expresses ratiometric Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.
• Depositing Lab: Samie Jaffrey
• Publication: Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038/s41589-021-00925-0. doi: 10.1038/s41589-021-00925-0.

Backbone

• Vector backbone: pAV-U6+27
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Tornado-F30-Broccoli-Squash-SAM-sensor 4-2
• Species: Synthetic
• Promoter: U6+27

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: 5'-GAGGGCCTATTTCCCATGATT-3'
• 3′ sequencing primer: 5'-CATATGGATATCGGTAAC-3'

Resource Information

• Supplemental Documents:
  • pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 4-2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene comment: Please note that the Addgene verified sequence differs from the depositor's original plasmid map and .gb file. Please refer to the Addgene sequence for the most accurate results. The depositor's map is still shown since it contains useful annotations.