pAV-U6+27-Tornado-F30-Squash-Broccoli
(Plasmid #177918)

• Purpose: Expresses a bifunctional circular RNA with Squash in one arm and Broccoli in the other arm of the F30 scaffold, in mammalian cells, using the Tornado expression system.
• Depositing Lab: Samie Jaffrey
• Publication: Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038/s41589-021-00925-0. doi: 10.1038/s41589-021-00925-0.

Backbone

• Vector backbone: pAV-U6+27
• Backbone size w/o insert (bp): 6151
• Total vector size (bp): 6480
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Tornado-F30-Squash-Broccoli
• Species: Synthetic
• Insert Size (bp): 329
• Promoter: U6+27

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: 5'-GAGGGCCTATTTCCCATGATT-3'
• 3′ sequencing primer: 5'-CATATGGATATCGGTAAC-3'

Resource Information

• Supplemental Documents:
  • pAV-U6+27-Tornado-F30-Squash-Broccoli
  • pAV-U6+27-Tornado-F30-Squash-Broccoli

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry