pAV-U6+27-Tornado-Squash-SAM-sensor 5-1
(Plasmid #177917)

• Purpose: Expresses Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system.
• Depositing Lab: Samie Jaffrey
• Publication: Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038/s41589-021-00925-0. doi: 10.1038/s41589-021-00925-0.

Backbone

• Vector backbone: pAV-U6+27
• Backbone size w/o insert (bp): 6150
• Total vector size (bp): 6419
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Tornado-Squash-SAM-sensor 5-1
• Species: Synthetic
• Insert Size (bp): 269
• Promoter: U6+27

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: 5'-GAGGGCCTATTTCCCATGATT-3'
• 3′ sequencing primer: 5'-CATATGGATATCGGTAAC-3'

Resource Information

• Supplemental Documents:
  • pAV-U6+27-Tornado-Squash-SAM-sensor 5-1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry