pCMV-FRB-tTA-Myc
(Plasmid
#177911)
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PurposeFRB-tTA-Myc tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177911 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFRB-tTA
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SpeciesSynthetic
- Promoter CMV promoter
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Tag
/ Fusion Protein
- Myc tag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Bglob-intron-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-FRB-tTA-Myc was a gift from Masaharu Somiya (Addgene plasmid # 177911 ; http://n2t.net/addgene:177911 ; RRID:Addgene_177911) -
For your References section:
Engineering of Extracellular Vesicles for Small Molecule-Regulated Cargo Loading and Cytoplasmic Delivery of Bioactive Proteins. Somiya M, Kuroda S. Mol Pharm. 2022 May 20. doi: 10.1021/acs.molpharmaceut.2c00192. 10.1021/acs.molpharmaceut.2c00192 PubMed 35594496
