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Addgene

GFP-STONIN2
(Plasmid #177885)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177885 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 7400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    stonin2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2700
  • Entrez Gene
    STON2 (a.k.a. STN2, STNB, STNB2)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde1 (unknown if destroyed)
  • 3′ cloning site Xma1 (unknown if destroyed)
  • 5′ sequencing primer GFP-FW
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-STONIN2 was a gift from Juan Bonifacino (Addgene plasmid # 177885 ; http://n2t.net/addgene:177885 ; RRID:Addgene_177885)

  • For your References section:

    Stonin 2: an adaptor-like protein that interacts with components of the endocytic machinery. Martina JA, Bonangelino CJ, Aguilar RC, Bonifacino JS. J Cell Biol. 2001 May 28;153(5):1111-20. doi: 10.1083/jcb.153.5.1111. 10.1083/jcb.153.5.1111 PubMed 11381094
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