DL034:cFos::HaloTag-iPAK4
(Plasmid
#177884)
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PurposeHaloTag-iPAK4 fusion with cFos promoter, cloned into a lentivirus backbone
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177884 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLenti cFos
- Total vector size (bp) 11591
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameiPAK4
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SpeciesSynthetic
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Insert Size (bp)1974
- Promoter cFos
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Tag
/ Fusion Protein
- HaloTag (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer AAGCTTTCCTTTAGGAACAGAG
- 3′ sequencing primer GATATCGAATTCTCATCTGGTGCG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DL034:cFos::HaloTag-iPAK4 was a gift from Adam Cohen (Addgene plasmid # 177884 ; http://n2t.net/addgene:177884 ; RRID:Addgene_177884) -
For your References section:
Time-tagged ticker tapes for intracellular recordings. Lin D, Li X, Moult E, Park P, Tang B, Shen H, Grimm JB, Falco N, Jia BZ, Baker D, Lavis LD, Cohen AE. Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01524-7. 10.1038/s41587-022-01524-7 PubMed 36593408
