pUS200
(Plasmid
#17788)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17788 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEMBL8+
- Backbone size w/o insert (bp) 3939
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCytochrome P450cam
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Alt nameCYP101
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SpeciesP. putida
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Insert Size (bp)1578
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GenBank IDM12546
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PstI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer M13rev
- 3′ sequencing primer M13for (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUS200 was a gift from Stephen Sligar (Addgene plasmid # 17788 ; http://n2t.net/addgene:17788 ; RRID:Addgene_17788) -
For your References section:
Nucleotide sequence of the Pseudomonas putida cytochrome P-450cam gene and its expression in Escherichia coli. Unger BP, Gunsalus IC, Sligar SG. J Biol Chem. 1986 Jan 25. 261(3):1158-63. PubMed 3003058