Rosa26 mT/mG
(Plasmid #17787)

• Depositing Lab: Liqun Luo
• Publication: Muzumdar et al Genesis. 2007 Sep . 45(9):593-605.

Backbone

• Vector backbone: pBluescript
• Backbone size w/o insert (bp): 4969
• Vector type: Mammalian Expression, Mouse Targeting, Cre/Lox
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rosa26 mT/mG
• Alt name: TdTomato
• Alt name: mT
• Alt name: EGFP
• Species: Aquorea victoria
• Insert Size (bp): 5500
• Tag / Fusion Protein:
  • membrane tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: See map (not destroyed)
• 3′ cloning site: See map (not destroyed)
• 5′ sequencing primer: See map

Resource Information

• Addgene Notes:
  • GenBank annotation for Rosa26 mT/mG
• Articles Citing this Plasmid:
  • 20 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See the 'Notes' section for the annotated GenBank file. Please note that some portions of the sequence (e.g., Rosa26 targeting arms and CA promoter) may not be entirely accurate.