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pEXSISERS_NLuc_FRT-PuroR
(Plasmid #177865)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177865 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBlueScript-KS II (+)
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology, Unspecified
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLuc-based EXSISERS
  • Species
    H. sapiens (human), S. cerevisiae (budding yeast), Synthetic
  • Tag / Fusion Protein
    • OLLAS

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HMGU has filed a patent (WO2020161236A1 “Detection of splice events of a gene of interest”) disclosing the capabilities of EXSISERS, and retains ownership rights to EXSISERS Material.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEXSISERS_NLuc_FRT-PuroR was a gift from Gil Westmeyer (Addgene plasmid # 177865 ; http://n2t.net/addgene:177865 ; RRID:Addgene_177865)

  • For your References section:

    Non-invasive and high-throughput interrogation of exon-specific isoform expression. Truong DJ, Phlairaharn T, Esswein B, Gruber C, Tumen D, Baligacs E, Armbrust N, Vaccaro FL, Lederer EM, Beck EM, Geilenkeuser J, Goppert S, Krumwiede L, Gratz C, Raffl G, Schwarz D, Zirngibl M, Zivanic M, Beyer M, Korner JD, Santl T, Evsyukov V, Strauss T, Schwarz SC, Hoglinger GU, Heutink P, Doll S, Conrad M, Giesert F, Wurst W, Westmeyer GG. Nat Cell Biol. 2021 Jun;23(6):652-663. doi: 10.1038/s41556-021-00678-x. Epub 2021 Jun 3. 10.1038/s41556-021-00678-x PubMed 34083785
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