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Addgene

pSumo-His6- Sumo-AtTPL(1-209)
(Plasmid #177856)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177856 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSUMO
  • Backbone manufacturer
    Life Sensors
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TPL
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    TPL (a.k.a. AT1G15750, F7H2.9, F7H2_9, TOPLESS, WSIP1, WUS-INTERACTING PROTEIN 1)
  • Tag / Fusion Protein
    • 6xHis-SUMO (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7Prom
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSumo-His6- Sumo-AtTPL(1-209) was a gift from Karsten Melcher (Addgene plasmid # 177856 ; http://n2t.net/addgene:177856 ; RRID:Addgene_177856)

  • For your References section:

    Structural basis for recognition of diverse transcriptional repressors by the TOPLESS family of corepressors. Ke J, Ma H, Gu X, Thelen A, Brunzelle JS, Li J, Xu HE, Melcher K. Sci Adv. 2015 Jul 24;1(6):e1500107. doi: 10.1126/sciadv.1500107. eCollection 2015 Jul. 10.1126/sciadv.1500107 PubMed 26601214
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