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Addgene

pSUMO His6 SUMO AMPKa (11-281)
(Plasmid #177851)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177851 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSUMO
  • Backbone manufacturer
    Life Sensors
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AMPK a1
  • Species
    H. sapiens (human)
  • Entrez Gene
    PRKAA1 (a.k.a. AMPK, AMPK alpha 1, AMPKa1)
  • Tag / Fusion Protein
    • 6xHis-SUMO (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUMO His6 SUMO AMPKa (11-281) was a gift from Karsten Melcher (Addgene plasmid # 177851 ; http://n2t.net/addgene:177851 ; RRID:Addgene_177851)

  • For your References section:

    Structural basis of AMPK regulation by adenine nucleotides and glycogen. Li X, Wang L, Zhou XE, Ke J, de Waal PW, Gu X, Tan MH, Wang D, Wu D, Xu HE, Melcher K. Cell Res. 2015 Jan;25(1):50-66. doi: 10.1038/cr.2014.150. Epub 2014 Nov 21. 10.1038/cr.2014.150 PubMed 25412657
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