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Addgene

pSUMO His6 SUMO SnRK2.3 (19-361)
(Plasmid #177846)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177846 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSUMO
  • Backbone manufacturer
    Life Sensors
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SNF1-related protein kinase 2.3
  • Alt name
    SRK2I
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    SNRK2.3 (a.k.a. AT5G66880, SNRK2-3, SRK2I, SUCROSE NONFERMENTING 1 (SNF1)-RELATED PROTEIN KINASE 2-3, sucrose nonfermenting 1(SNF1)-related protein kinase 2.3)
  • Tag / Fusion Protein
    • 6xHis-SUMO (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUMO His6 SUMO SnRK2.3 (19-361) was a gift from Karsten Melcher (Addgene plasmid # 177846 ; http://n2t.net/addgene:177846 ; RRID:Addgene_177846)

  • For your References section:

    Structural basis for basal activity and autoactivation of abscisic acid (ABA) signaling SnRK2 kinases. Ng LM, Soon FF, Zhou XE, West GM, Kovach A, Suino-Powell KM, Chalmers MJ, Li J, Yong EL, Zhu JK, Griffin PR, Melcher K, Xu HE. Proc Natl Acad Sci U S A. 2011 Dec 27;108(52):21259-64. doi: 10.1073/pnas.1118651109. Epub 2011 Dec 12. 10.1073/pnas.1118651109 PubMed 22160701
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