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Addgene

pCDNA6-IgK-hFRa(23-234)C11S-th-Fc
(Plasmid #177841)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177841 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCDNA6
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Folate receptor alpha
  • Species
    H. sapiens (human)
  • Mutation
    all 11 C mutated to S
  • Entrez Gene
    FOLR1 (a.k.a. FBP, FOLR, FRalpha, NCFTD)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

IgK = Ig kappa leader, th = thrombin cleavage site, Fc = Igl Fc fragment

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA6-IgK-hFRa(23-234)C11S-th-Fc was a gift from Karsten Melcher (Addgene plasmid # 177841 ; http://n2t.net/addgene:177841 ; RRID:Addgene_177841)
  • For your References section:

    Structural basis for molecular recognition of folic acid by folate receptors. Chen C, Ke J, Zhou XE, Yi W, Brunzelle JS, Li J, Yong EL, Xu HE, Melcher K. Nature. 2013 Aug 22;500(7463):486-9. doi: 10.1038/nature12327. Epub 2013 Jul 14. 10.1038/nature12327 PubMed 23851396