pK18msB
(Plasmid #177839)

• Purpose: (Empty Backbone) Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.
• Depositing Lab: Christopher Johnson
• Publication: Ling et al Nat Commun. 2022 Aug 22;13(1):4925. doi: 10.1038/s41467-022-32296-y.

Backbone

• Vector backbone: pK18sB
• Backbone size (bp): 3282
• Vector type: Bacterial gene replacement
• Selectable markers: Counterselection: Sucrose

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Special M13R binding site, M13R: CAGGAAACAGCTATGAC
• 3′ sequencing primer: M13F: TGTAAAACGACGGCCAGT

Resource Information

• Supplemental Documents:
  • pK18msB.gb
• A portion of this plasmid was derived from a plasmid made by: Sequences were derived from pK18mobsacB (ATCC 87097)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The sequence for this plasmid has been deposited with Genbank (Accession # OK423783).

Sequences were derived from pK18mobsacB (ATCC 87097), which was originally described in Schäfer, A., Tauch, A., Jäger, W., Kalinowski, J., Thierbach, G., Pühler, A., 1994. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145, 69–73. doi:10.1016/0378-1119(94)90324-7