pK18sB
(Plasmid #177838)

• Purpose: (Empty Backbone) Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.
• Depositing Labs: Gregg Beckham, Christopher Johnson
• Publication: Jayakody et al Energy Environ. Sci., 2018,11, 1625-1638

Backbone

• Vector backbone: pK18sB
• Backbone size (bp): 3038
• Vector type: Bacterial gene replacement
• Selectable markers: Counterselection: Sucrose

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Special M13R binding site, M13R: CAGGAAACAGCTATGAC
• 3′ sequencing primer: M13F: TGTAAAACGACGGCCAGT

Resource Information

• Supplemental Documents:
  • pK18sB.gb
• A portion of this plasmid was derived from a plasmid made by: Sequences were derived from pK18mobsacB (ATCC 87097).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The sequence for this plasmid has been deposited with Genbank (Accession # MH166772).

Sequences were derived from pK18mobsacB (ATCC 87097), which was originally described in Schäfer, A., Tauch, A., Jäger, W., Kalinowski, J., Thierbach, G., Pühler, A., 1994. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145, 69–73. doi:10.1016/0378-1119(94)90324-7