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Addgene

pBAD-HisD-KRaION2
(Plasmid #177800)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177800 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD-HisD
  • Backbone size w/o insert (bp) 4002
  • Total vector size (bp) 5175
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    KRaION2
  • Species
    Synthetic
  • Insert Size (bp)
    1173
  • GenBank ID
    OK187807
  • Promoter araB

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pBAD-Fw
  • 3′ sequencing primer pBAD-Rv
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.10.07.463355 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD-HisD-KRaION2 was a gift from Edward Boyden (Addgene plasmid # 177800 ; http://n2t.net/addgene:177800 ; RRID:Addgene_177800)

  • For your References section:

    Tuning the Sensitivity of Genetically Encoded Fluorescent Potassium Indicators through Structure-Guided and Genome Mining Strategies. Torres Cabán CC, Yang M, Lai C, Yang L, Subach FV, Smith BO, Piatkevich KD, Boyden ES. ACS Sens. 2022 May 27;7(5):1336-1346. doi: 10.1021/acssensors.1c02201. Epub 2022 Apr 15. 10.1021/acssensors.1c02201 PubMed 35427452
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