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Addgene

pDEST-DHFR F[1,2]-N (TRP1)
(Plasmid #177797)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177797 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST-AD-CYH2
  • Backbone size (bp) 10388
  • Modifications to backbone
    Removed AD and CYH2 used for Y2H, and inserted DHFR F[1,2] fragment. Inserted I-CeuI, I-SceI, and PI-PspI homing endonuclease sites on backbone.
  • Vector type
    Yeast Expression
  • Promoter ADH1
  • Selectable markers
    TRP1
  • Tag / Fusion Protein
    • DHFR F[1,2] (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-DHFR F[1,2]-N (TRP1) was a gift from Christian Landry (Addgene plasmid # 177797 ; http://n2t.net/addgene:177797 ; RRID:Addgene_177797)

  • For your References section:

    Barcode fusion genetics-protein-fragment complementation assay (BFG-PCA): tools and resources that expand the potential for binary protein interaction discovery. Evans-Yamamoto D, Rouleau FD, Nanda P, Makanae K, Liu Y, Despres PC, Matsuo H, Seki M, Dube AK, Ascencio D, Yachie N, Landry CR. Nucleic Acids Res. 2022 Feb 7. pii: 6523796. doi: 10.1093/nar/gkac045. 10.1093/nar/gkac045 PubMed 35137167
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