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Addgene

pdestMB14_sur5p-dCas9-VP64
(Plasmid #177788)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177788 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pdestMB14 (Addgene #26415)
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-VP64
  • Species
    C. elegans (nematode)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pdestMB14_sur5p-dCas9-VP64 was a gift from Michael Ristow (Addgene plasmid # 177788 ; http://n2t.net/addgene:177788 ; RRID:Addgene_177788)

  • For your References section:

    Ingestion of single guide RNAs induces gene overexpression and extends lifespan in C. elegans via CRISPR activation. Fischer F, Benner C, Goyala A, Grigolon G, Vitiello D, Wu J, Zarse K, Ewald CY, Ristow M. J Biol Chem. 2022 May 27:102085. doi: 10.1016/j.jbc.2022.102085. 10.1016/j.jbc.2022.102085 PubMed 35636511
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