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Addgene

L4440_BioBrick-hif-1-sgRNA-B
(Plasmid #177785)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177785 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    L4440_BioBrick-sgRNA
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hif-1
  • gRNA/shRNA sequence
    AAAATTTGAGCAACTCTCAGGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT and ACTTCTCAAAAAAATTATGGGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT
  • Species
    C. elegans (nematode)
  • Entrez Gene
    hif-1 (a.k.a. CELE_F38A6.3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    L4440_BioBrick-hif-1-sgRNA-B was a gift from Michael Ristow (Addgene plasmid # 177785 ; http://n2t.net/addgene:177785 ; RRID:Addgene_177785)

  • For your References section:

    Ingestion of single guide RNAs induces gene overexpression and extends lifespan in C. elegans via CRISPR activation. Fischer F, Benner C, Goyala A, Grigolon G, Vitiello D, Wu J, Zarse K, Ewald CY, Ristow M. J Biol Chem. 2022 May 27:102085. doi: 10.1016/j.jbc.2022.102085. 10.1016/j.jbc.2022.102085 PubMed 35636511
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