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Addgene

pGL4.27_ARE/NRF2-SPE
(Plasmid #177775)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177775 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL4.27 (Promega Corporation, #E8451)
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NRF2
  • Species
    H. sapiens (human)
  • Entrez Gene
    NFE2L2 (a.k.a. HEBP1, IMDDHH, NRF2, Nrf-2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4.27_ARE/NRF2-SPE was a gift from Michael Ristow (Addgene plasmid # 177775 ; http://n2t.net/addgene:177775 ; RRID:Addgene_177775)

  • For your References section:

    Grainyhead 1 acts as a drug-inducible conserved transcriptional regulator linked to insulin signaling and lifespan. Grigolon G, Araldi E, Erni R, Wu JY, Thomas C, La Fortezza M, Laube B, Pohlmann D, Stoffel M, Zarse K, Carreira EM, Ristow M, Fischer F. Nat Commun. 2022 Jan 10;13(1):107. doi: 10.1038/s41467-021-27732-4. 10.1038/s41467-021-27732-4 PubMed 35013237
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