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Addgene

PX459_GRHL1-exon5
(Plasmid #177763)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177763 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PX459 (Addgene #62988)
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GRHL1
  • gRNA/shRNA sequence
    TCCGCAGACTGAGATCCGAGgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgc
  • Species
    H. sapiens (human)
  • Entrez Gene
    GRHL1 (a.k.a. LBP32, MGR, NH32, TFCP2L2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PX459_GRHL1-exon5 was a gift from Michael Ristow (Addgene plasmid # 177763 ; http://n2t.net/addgene:177763 ; RRID:Addgene_177763)

  • For your References section:

    Grainyhead 1 acts as a drug-inducible conserved transcriptional regulator linked to insulin signaling and lifespan. Grigolon G, Araldi E, Erni R, Wu JY, Thomas C, La Fortezza M, Laube B, Pohlmann D, Stoffel M, Zarse K, Carreira EM, Ristow M, Fischer F. Nat Commun. 2022 Jan 10;13(1):107. doi: 10.1038/s41467-021-27732-4. 10.1038/s41467-021-27732-4 PubMed 35013237
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