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Addgene

CJ45 (miR-1003 5' splice site mut)
(Plasmid #17775)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17775 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMT-puro
  • Backbone manufacturer
    David Sabatini Lab
  • Vector type
    Insect Expression ; Drosophila metallothionein gene promoter drives expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dme-miR-1003 minigene 5' splice mut
  • Alt name
    miR-1003
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    390
  • Mutation
    5' splice site mut + compsensatory stem

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer MT forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Site-directed mutagenesis was used to make 5' splice site mutations with the indicated primers: GCTGCGCAGA ACGTGGGCAT CTGGATGTGG TTGGC and GCCAACCACA TCCAGATGCC CACGTTCTGC GCAGC; CCTCTCACAT TTACATGTTC ACAGGCGCCGT GAG and CTCACGGCGC CTGTGAACAT GTAAATGTGA GAGG.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CJ45 (miR-1003 5' splice site mut) was a gift from David Bartel (Addgene plasmid # 17775 ; http://n2t.net/addgene:17775 ; RRID:Addgene_17775)

  • For your References section:

    Intronic microRNA precursors that bypass Drosha processing. Ruby JG, Jan CH, Bartel DP. Nature. 2007 Jul 5. 448(7149):83-6. 10.1038/nature05983 PubMed 17589500
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