pCMV(MinDis)-iCytSnFR_PM
(Plasmid
#177743)
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PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177743 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV(MinDis)
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Backbone manufacturermodified from Invitrogen
- Backbone size w/o insert (bp) 5005
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepCMV(MinDis)-iCytSnFR_PM
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SpeciesSynthetic
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Insert Size (bp)1827
- Promoter hCMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGHR (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.10.04.463082v3.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV(MinDis)-iCytSnFR_PM was a gift from Henry Lester (Addgene plasmid # 177743 ; http://n2t.net/addgene:177743 ; RRID:Addgene_177743) -
For your References section:
Fluorescence activation mechanism and imaging of drug permeation with new sensors for smoking-cessation ligands. Nichols AL, Blumenfeld Z, Fan C, Luebbert L, Blom AE, Cohen BN, Marvin JS, Borden PM, Kim CH, Muthusamy AK, Shivange AV, Knox HJ, Campello HR, Wang JH, Dougherty DA, Looger LL, Gallagher T, Rees DC, Lester HA. eLife. 2022 Jan 4;11. pii: 74648. doi: 10.7554/eLife.74648. 10.7554/eLife.74648 PubMed 34982029
