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Addgene

iCyt_F_SnFR
(Plasmid #177739)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177739 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHHM.X51
  • Backbone manufacturer
    Caltech
  • Backbone size w/o insert (bp) 2761
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iCyt_F_SnFR
  • Species
    Synthetic
  • Insert Size (bp)
    1683
  • Promoter T7
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • His (N terminal on insert)
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    iCyt_F_SnFR was a gift from Henry Lester (Addgene plasmid # 177739 ; http://n2t.net/addgene:177739 ; RRID:Addgene_177739)

  • For your References section:

    Fluorescence activation mechanism and imaging of drug permeation with new sensors for smoking-cessation ligands. Nichols AL, Blumenfeld Z, Fan C, Luebbert L, Blom AE, Cohen BN, Marvin JS, Borden PM, Kim CH, Muthusamy AK, Shivange AV, Knox HJ, Campello HR, Wang JH, Dougherty DA, Looger LL, Gallagher T, Rees DC, Lester HA. eLife. 2022 Jan 4;11. pii: 74648. doi: 10.7554/eLife.74648. 10.7554/eLife.74648 PubMed 34982029
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