pLVX-Egr2ASRNA
(Plasmid
#177737)
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PurposeEgr2 AS-RNA lentiviral expression vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 177737 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLVX-DsRed-Express2-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 8745
- Total vector size (bp) 9741
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEgr2-AS-RNA
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Alt nameAntisense of the Egr2 proximal promoter
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SpeciesM. musculus (mouse), R. norvegicus (rat)
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Insert Size (bp)966
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVX-Egr2ASRNA was a gift from Nikolaos Tapinos (Addgene plasmid # 177737 ; http://n2t.net/addgene:177737 ; RRID:Addgene_177737) -
For your References section:
Regulation of Peripheral Myelination through Transcriptional Buffering of Egr2 by an Antisense Long Non-coding RNA. Martinez-Moreno M, O'Shea TM, Zepecki JP, Olaru A, Ness JK, Langer R, Tapinos N. Cell Rep. 2017 Aug 22;20(8):1950-1963. doi: 10.1016/j.celrep.2017.07.068. 10.1016/j.celrep.2017.07.068 PubMed 28834756