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p406 – TDH3-ER-FlucDM-GFP11-Ura
(Plasmid #177731)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177731 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p406 TDH3
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FlucDM
  • Mutation
    R188Q, R261Q (Destabilized Mutant)
  • Tags / Fusion Proteins
    • KAR2 Signal Sequence (N terminal on insert)
    • HA (C terminal on insert)
    • GFP11 (C terminal on insert)
    • HDEL (C terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p406 – TDH3-ER-FlucDM-GFP11-Ura was a gift from Erik Böttger (Addgene plasmid # 177731 ; http://n2t.net/addgene:177731 ; RRID:Addgene_177731)

  • For your References section:

    ER-misfolded proteins become sequestered with mitochondria and impair mitochondrial function. Cortes Sanchon A, Santhosh Kumar H, Mantovani M, Osinnii I, Mateos JM, Kaech A, Shcherbakov D, Akbergenov R, Bottger EC. Commun Biol. 2021 Dec 2;4(1):1350. doi: 10.1038/s42003-021-02873-w. 10.1038/s42003-021-02873-w PubMed 34857875
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