Skip to main content
Addgene

pPLN-hFluc-DM-eGFP-KDEL
(Plasmid #177728)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177728 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    phFluc-WT
  • Backbone manufacturer
    Addgene #177714
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hFlucDM
  • Insert Size (bp)
    2560
  • Mutation
    R188Q, R261Q (Destabilized Mutant)
  • Tags / Fusion Proteins
    • Prolactin Signal Sequence (N terminal on insert)
    • eGFP (C terminal on insert)
    • KDEL (C terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPLN-hFluc-DM-eGFP-KDEL was a gift from Erik Böttger (Addgene plasmid # 177728 ; http://n2t.net/addgene:177728 ; RRID:Addgene_177728)

  • For your References section:

    ER-misfolded proteins become sequestered with mitochondria and impair mitochondrial function. Cortes Sanchon A, Santhosh Kumar H, Mantovani M, Osinnii I, Mateos JM, Kaech A, Shcherbakov D, Akbergenov R, Bottger EC. Commun Biol. 2021 Dec 2;4(1):1350. doi: 10.1038/s42003-021-02873-w. 10.1038/s42003-021-02873-w PubMed 34857875
Related items: