pPLN-hFluc-WT-HA-GFP11-KDEL
(Plasmid
#177722)
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PurposeFirefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177722 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonephFluc-WT
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Backbone manufacturerAddgene #177714
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Vector typeMammalian Expression, Bacterial Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehFluc
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Insert Size (bp)1947
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Tags
/ Fusion Proteins
- Prolactin Signal Sequence (N terminal on insert)
- HA (C terminal on insert)
- GFP11 (C terminal on insert)
- KDEL (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPLN-hFluc-WT-HA-GFP11-KDEL was a gift from Erik Böttger (Addgene plasmid # 177722 ; http://n2t.net/addgene:177722 ; RRID:Addgene_177722) -
For your References section:
ER-misfolded proteins become sequestered with mitochondria and impair mitochondrial function. Cortes Sanchon A, Santhosh Kumar H, Mantovani M, Osinnii I, Mateos JM, Kaech A, Shcherbakov D, Akbergenov R, Bottger EC. Commun Biol. 2021 Dec 2;4(1):1350. doi: 10.1038/s42003-021-02873-w. 10.1038/s42003-021-02873-w PubMed 34857875
