phFluc-WT
(Plasmid
#177714)
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PurposeCytosolic firefly luciferase reporter previously described in Shcherbakov et al. 2019
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 177714 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL4.14 (intermed. pUC19-construct)
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Vector typeMammalian Expression, Bacterial Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehFluc WT
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Insert Size (bp)1653
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
phFluc-WT was a gift from Erik Böttger (Addgene plasmid # 177714 ; http://n2t.net/addgene:177714 ; RRID:Addgene_177714) -
For your References section:
Ribosomal mistranslation leads to silencing of the unfolded protein response and increased mitochondrial biogenesis. Shcherbakov D, Teo Y, Boukari H, Cortes-Sanchon A, Mantovani M, Osinnii I, Moore J, Juskeviciene R, Brilkova M, Duscha S, Kumar HS, Laczko E, Rehrauer H, Westhof E, Akbergenov R, Bottger EC. Commun Biol. 2019 Oct 17;2:381. doi: 10.1038/s42003-019-0626-9. eCollection 2019. 10.1038/s42003-019-0626-9 PubMed 31637312