LT2_14_GlyOx
(Plasmid #177711)

• Purpose: Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)
• Depositing Lab: Lisbeth Olsson
• Publication: Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. doi: 10.3389/fmicb.2021.802169. eCollection 2021.

Backbone

• Vector backbone: Empty backbone
• Total vector size (bp): 6932
• Vector type: Yeast Expression ; Used as donor DNA in genome integration after linearisation with NotI

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: Grow bacteria at 30°C to reduce recombination events
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
• Species: S. cerevisiae (budding yeast)

Gene/Insert 2

• Gene/Insert name: pTEFmut8-mCherry-tADH1
• Species: S. cerevisiae (budding yeast)

Gene/Insert 3

• Gene/Insert name: pTRX2_5xUAS-ymYPET-tCYC1
• Species: S. cerevisiae (budding yeast)

Resource Information

• Supplemental Documents:
  • LT2_14_GlyOx.gb
• A portion of this plasmid was derived from a plasmid made by: Sequence for ymYPET obtained from Addgene plasmid #118455; Sequence for pTRX2_5xUAS amplified from Addgene plasmid #124708; Sequence for pTEFmut8 amplified from Addgene plasmid #124582; Sequence for RNA_2.6 obtained from Addgene plasmid #162800

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry