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Addgene

LT1_36_QUEEN-2m
(Plasmid #177708)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177708 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Empty backbone
  • Total vector size (bp) 4590
  • Vector type
    Yeast Expression ; Used as donor DNA in genome integration after linearisation with NotI

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pTEF1-QUEEN2m
  • Species
    S. cerevisiae (budding yeast)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Digital CDS of QUEEN-2m was obtained from Addgene plasmid #129350

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LT1_36_QUEEN-2m was a gift from Lisbeth Olsson (Addgene plasmid # 177708 ; http://n2t.net/addgene:177708 ; RRID:Addgene_177708)

  • For your References section:

    Real-Time Monitoring of the Yeast Intracellular State During Bioprocesses With a Toolbox of Biosensors. Torello Pianale L, Rugbjerg P, Olsson L. Front Microbiol. 2022 Jan 7;12:802169. doi: 10.3389/fmicb.2021.802169. eCollection 2021. 10.3389/fmicb.2021.802169 PubMed 35069506
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