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Addgene

A0A075HNX4
(Plasmid #177695)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177695 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Backbone size w/o insert (bp) 5361
  • Total vector size (bp) 6825
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Long chain alcohol oxidase
  • Alt name
    LCAO
  • Species
    uncultured marine group II/III euryarchaeote KM3_72_H01
  • Insert Size (bp)
    1464
  • GenBank ID
    AIF16132
  • Promoter T7
  • Tag / Fusion Protein
    • 10xHis (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Twist Bioscience

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    A0A075HNX4 was a gift from Martin Engqvist (Addgene plasmid # 177695 ; http://n2t.net/addgene:177695 ; RRID:Addgene_177695)

  • For your References section:

    Discovery of Two Novel Oxidases Using a High-Throughput Activity Screen. Rembeza E, Boverio A, Fraaije MW, Engqvist MKM. Chembiochem. 2022 Jan 19;23(2):e202100510. doi: 10.1002/cbic.202100510. Epub 2021 Nov 18. 10.1002/cbic.202100510 PubMed 34709726
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