A3RXB7
(Plasmid #177694)

• Purpose: expresses a N-acetyl hexosamine oxidase in E. coli
• Depositing Lab: Martin Engqvist
• Publication: Rembeza et al Chembiochem. 2022 Jan 19;23(2):e202100510. doi: 10.1002/cbic.202100510. Epub 2021 Nov 18.

Backbone

• Vector backbone: pET21a
• Backbone size w/o insert (bp): 5361
• Total vector size (bp): 6912
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: N-acetylhexosamine oxidase
• Alt name: HexNACO
• Species: Ralstonia solanacearum UW551
• Insert Size (bp): 1551
• GenBank ID: RRSL_02030
• Promoter: T7
• Tag / Fusion Protein:
  • 10xHis (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: TwistBioscience

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry