1a72
(Plasmid
#177690)
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PurposeHCV E1E2 expression plasmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 177690 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.2/V5/Dest
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7711
- Total vector size (bp) 9416
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHCV E1E2
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SpeciesHepatitis C virus
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Insert Size (bp)1705
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GenBank IDKJ187977
- Promoter CMV
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer na
- 3′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
1a72 was a gift from Justin Bailey (Addgene plasmid # 177690 ; http://n2t.net/addgene:177690 ; RRID:Addgene_177690) -
For your References section:
An Antigenically Diverse, Representative Panel of Envelope Glycoproteins for Hepatitis C Virus Vaccine Development. Salas JH, Urbanowicz RA, Guest JD, Frumento N, Figueroa A, Clark KE, Keck Z, Cowton VM, Cole SJ, Patel AH, Fuerst TR, Drummer HE, Major M, Tarr AW, Ball JK, Law M, Pierce BG, Foung SKH, Bailey JR. Gastroenterology. 2022 Feb;162(2):562-574. doi: 10.1053/j.gastro.2021.10.005. Epub 2021 Oct 13. 10.1053/j.gastro.2021.10.005 PubMed 34655573